RNA interference mediated knockdown of Brugia malayi UDP-Galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae

Table 6 Percentage reduction in motility of microfilariae released by B. malayi female adult parasites after in vitro RNA interference was analyzed by scoring their motility

Time (h)	Reduction in microfilaria motility^a
Time (h)	MC	SCR	siRNA1	siRNA 2	siRNA1 + 2
24	5+	5+	2+	2+	1+
48	5+	5+	1+	1+	1+
72	5+	5+	1+/D	1+/D	1+/D
96	5+	5+	1+/D	1+/D	1+/D
120	5+	5+	1+/D	1+/D	1+/D

Abbreviations: MC medium control; SCR negative control (scrambled siRNA)
^a5, 0% motility reduction; 4, 1–25% reduction; 3, 26–49% reduction; 2, 50–74% reduction; 1, 75–99% reduction; D (death), 100% reduction

ISSN: 1756-3305