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Table 2 Primers for amplification and sequencing of the ribosomal transcription unit

From: The ribosomal transcription units of Haplorchis pumilio and H. taichui and the use of 28S rDNA sequences for phylogenetic identification of common heterophyids in Vietnam

Primer name

Sequence (5'–3')

Length (bp)

Tm (°C)

Target gene

Reference

UD18SF

AACCTGGTTGATCCTGCCAG

20

59

18S (F)

[15]

NS1F

GTAGTCATATGCTTGTCTC

19

48

18S (F)

This study

U18SF

GCGAATGGCTCATTAAATCAGC

22

57

18S (F)

This study

U18S2Ra

GGTTCTGTTCTAATAAATCCAC

22

50

18S (R)

This study

U18SARa

CCGTCGCCGACACAAGGCCGAC

22

67

18S (R)

This study

NS2Fa

GCAAGTCTGGTGCCAGCAGCC

21

66

18S (F)

This study

NS2Ra

GGCCTGCTTTGAGCACTC

18

59

18S (R)

This study

U18S2F

TCGTGACTGGGATCGGGGC

19

64

18S (F)

This study

NS5Fa

TGAATGGTTTAGCAAGGTCCTCGG

24

61

18S (F)

This study

U18SRa

GGAACCAATCCGAGGACCTTGC

22

63

18S (R)

This study

NS8Ra

CACCTACGGAAACCTTGTTACGACTT

26

60

18S (R)

This study

U3SF

GGTACCGGTGGATCACTCGGCTCGTG

26

67

5.8S (F)

This study

U3SR

CGACCCTCGGACAGGCG

17

64

5.8S (R)

This study

U28SFa

CTAACAAGGATTCCCTTAGTAAC

23

52

28S (F)

This study

U28S2Ra

ACAACCCGACTCCAAGGTC

19

59

28S (R)

This study

U28Fa

TCGGAGACGGCGGCTTG

17

63

28S (F)

This study

U28S2Fa

ATCACCGGCCCGTCCCATG

19

65

28S (F)

This study

U28SR

GTCTTTCGCCCCTATACTCAC

21

57

28S (R)

This study

1500R

GCTATCCTGAGGGAAACTTCG

21

57

28S (R)

[15]

  1. Abbreviations F forward, R reverse, Tm melting temperature
  2. aPrimers used for sequencing