Table 4 Position of ribosomal genes and internal transcribed spacers in the partially sequenced transcription unit of Haplorchis pumilio (4,943Â bp) and H. taichui (4,796Â bp)
Gene/region | Position (5'–3') | Repeat | Size (bp) | Intergenic spacer (bp) | Note |
|---|---|---|---|---|---|
H. pumilio | Â | Â | Â | Â | GenBank: KX815125 |
18S | 1–1992 |  | 1,992 | 0 |  |
ITS1 | 1993–3098 |  | 1,106 | +66 | 66 bp to TRA1 |
|  | 2059–2194 | TRA1 | 136 | 0 | Tandem |
|  | 2195–2330 | TRA2 | 136 | 0 | Tandem |
|  | 2331–2466 | TRA3 | 136 | -91 | Overlap with TRB1 |
|  | 2376–2498 | TRB1 | 123 | 0 | Tandem |
|  | 2499–2621 | TRB2 | 123 | 0 | Tandem |
|  | 2622–2705 | TRB3 (partial) | 84 | +393 | 393 bp to 5.8S |
5.8S | 3099–3258 |  | 160 | 0 |  |
ITS2 | 3259–3546 |  | 288 | 0 | No repeats |
28S | 3547–4943 |  | 1,397 |  | 5' partial sequence |
H. taichui | Â | Â | Â | Â | GenBank: KX815126 |
18S | 1–1992 |  | 1,992 | 0 |  |
ITS1 | 1993–2779 |  | 797 | 0 | No repeats |
5.8S | 2790–2949 |  | 160 | 0 |  |
ITS2 | 2950–3393 |  | 444 | +121 | 121 bp to TR1 |
|  | 3071–3155 | TR1 | 85 | 0 | Tandem |
|  | 3156–3238 | TR2 | 83 | 0 | Tandem |
|  | 3239–3323 | TR3 | 85 | +70 | Tandem |
28S | 3394–4796 |  | 1,403 |  | 5' partial sequence |