Fig. 2
From: Laboratory confirmed miltefosine resistant cases of visceral leishmaniasis from India
PCR analysis of buffy coat sample from serologically positive VL patients. Specific PCR product for ITS region of Leishmania spp. (~1.1Â kb product; arrow). Lanes 1, 2: PCR amplified products of DNA extracted from the buffy coat of LD843 and LD860 patients respectively; Lane 3: positive control (DNA extracted from DD8 strain of L. donovani promastigotes); Lane 4: positive control (DNA extracted from the buffy coat of known positive patient); Lane 5: negative control (DNA from the buffy coat of healthy individual); Lane 6: 1Â kb molecular weight DNA ladder