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Fig. 2 | Parasites & Vectors

Fig. 2

From: Ultra-sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito-borne viruses and parasites

Fig. 2

Amplification of serially diluted VLPs by both RT-PCR and PCR. a The layout of the MBVs hybridization capture-chip. The probe P is the QC probe. The probe NC is the negative control probe. b Amplifications of serially diluted VLPs by both RT-PCR and PCR (both based on the GSPs of RT-PCR system A or B). The results showed that although DNase I did not completely digest DNA templates in high concentration VLPs, the extraction of low-concentration VLPs were only detected by RT-PCR and not by PCR, demonstrating that DNA templates had been diluted to sufficiently. c Principle of the CL imaging DNA hybridization method. Step 1 shows that capture probes are fixed to the aldehyde-chip surface. Step 2 shows that the denatured RT-PCR products are hybridized on the capture-chip. Steps 3–5 show the principle of CL detection. Biotin is incorporated into reverse strand in RT-PCR amplification. When HRP modified streptavidin is bound, CL signal becomes illuminant by the catalyzed substrates

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