Ultra-sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito-borne viruses and parasites

Table 2 The sequences of GSPs for multiplex asymmetric RT-PCR and PCR amplification

Pathogen	Primer	Sequence (5′ to 3′)	Location	Reference^a	Primer source
Dengue virus	DC10418	TTGAGTAAACYRTGCTGCCTGTAGCTC	10,392–10,481	GQ398257	[21]
JEV	JEV-3UTR–F2	GGACTGGGTTAACAAATCTG	10,525–10,544	EF107523	Present study
YFV	YEF-3UTR-F1	AAACTACGGATGGAGAACCG	10,489–10,508	U54798	Present study
WNV	WNV-3UTR-F2	AAGTTGAGTAGACGGTGCTGC	10,533–10,553	EU249803	Present study
SLEV	SLE-3UTR-F1	GGATGTCAGGTAAACGGTGCT	10,451–10,471	FJ753286	Present study
Flavivirus	CDC10564^b	GGGTCTCCTCTAACCTCTAGTCCT	10,569–10,592	GQ398257	[21]
EEEV	EEE-E1-F	ACACTAAATTCACCCTAGTTCGAT	11,376–11,399	AY705241	[22]
EEEV	EEE-1-R^b	GTGTATAAAATTACTTAGGAGCAGCATTATG	11,492–11,522	AY705241	[22]
CHIKV	CHIKV-F1	TGGATGAACATGGAAGTGAA	7,121–7,140	DQ443544	Present study
CHIKV	CHIKV-R2^b	GCTGTAGTGCGTACCTATTT	7,496–7,515	DQ443544	Present study
WEEV	WEE-F	AGGGATACCCCCGAAGGTT	8,220–8,238	AF214040	[23]
WEEV	WEE-R^b	GTGAATAGCACACGGGTGGTT	8,302–8,322	AF214040	[23]
VEEV	VEEV-cap-F3	GGGCGGCGCATGAGAGAAGC	3–22	U34999	Present study
VEEV	VEEV-cap-R3^b	GTGACCTGCTTGGCTTCTACCTC	119–141	U34999	Present study
Plasmodium	MAL-F1	GGGAGTGAAGACGATCAGATACC	443–465	HQ283215	Present study
Plasmodium	MAL-R1^b	CCGTGTTGAGTCAAATTAAGCC	668–689	HQ283215	Present study
W. bancrofti	BM/WBF	AGCGTGATGGCATCAAAGTAG	1–21	L20344	[24]
W. bancrofti	WBR^b	AGGTTATACCAAGCAAACAAAAA	110–132	L20344	[24]
B. malayi/ B. timori	MGB-HhaI-For	GCAATATACGACCAGCAC	254–271	M12691	[25]
B. malayi/ B. timori	MGB-HhaI-Rev^b	ACATTAGACAAGGAAATTGGTT	140–161	M12691	[25]

ISSN: 1756-3305