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Table 4 The copy numbers of in vitro transcribed RNAs quantified by UV spectrophotometer

From: Ultra-sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito-borne viruses and parasites

Virus RNA concentration (ng/μl)a Length (nt)b Copy number (copies/μl) c Location Referenced
SLEV 61 680 2 × 1011 10,451–10,933 FJ753286
WNV 550 654 1 × 1012 10,521–10,977 EU249803
VEEV 275 339 1 × 1012 1–142 U34999
JEV 1810 560 5 × 1012 10,525–10,887 EF107523
YFV 1930 853 4 × 1012 10,109–10,764 U54798
EEEV 1290 344 6 × 1012 11,376–11,522 AY705241
CHIKV 1700 590 5 × 1012 7,123–7,515 DQ443544
WEEV 510 303 4 × 1012 8,217–8,322 AF214040
DENV2 275 395 1 × 1012 10,392–10,589 GQ398257
  1. aRNA concentrations were quantified by UV spectrophotometer
  2. bThe lengths of in vitro transcribed RNAs were calculated on the length of the inserted gene fragments plus the transcript length of digested PGM-T vector
  3. cCopy numbers (copies/μl) = RNA concentration (ng/μl) × Avogadro constant × 10-9/ (RNA length × 340 g/mol)
  4. dGenBank accession numbers