Ultra-sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito-borne viruses and parasites

Table 4 The copy numbers of in vitro transcribed RNAs quantified by UV spectrophotometer

Virus	RNA concentration (ng/μl)^a	Length (nt)^b	Copy number (copies/μl) ^c	Location	Reference^d
SLEV	61	680	2 × 10¹¹	10,451–10,933	FJ753286
WNV	550	654	1 × 10¹²	10,521–10,977	EU249803
VEEV	275	339	1 × 10¹²	1–142	U34999
JEV	1810	560	5 × 10¹²	10,525–10,887	EF107523
YFV	1930	853	4 × 10¹²	10,109–10,764	U54798
EEEV	1290	344	6 × 10¹²	11,376–11,522	AY705241
CHIKV	1700	590	5 × 10¹²	7,123–7,515	DQ443544
WEEV	510	303	4 × 10¹²	8,217–8,322	AF214040
DENV2	275	395	1 × 10¹²	10,392–10,589	GQ398257

^aRNA concentrations were quantified by UV spectrophotometer
^bThe lengths of in vitro transcribed RNAs were calculated on the length of the inserted gene fragments plus the transcript length of digested PGM-T vector
^cCopy numbers (copies/μl) = RNA concentration (ng/μl) × Avogadro constant × 10^-9/ (RNA length × 340 g/mol)
^dGenBank accession numbers

ISSN: 1756-3305