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Table 5 Cut-off values of the capture probes

From: Ultra-sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito-borne viruses and parasites

Probe Average of negative sample (grey value) SD
(grey value)
Cut-off valuea
(grey value)
DEN-10731 0.50 0.67 2.51
DEN1-X10830 0.18 0.33 1.17
DEN2-X10831 0.85 1.55 5.50
DEN3-X10829 0.38 0.29 1.25
DEN4-X10714 0.15 0.22 0.81
JEV-10726 0.42 0.30 1.32
YFV-10679 1.90 3.80 13.30
WNV-10768 0.30 0.39 1.47
SLE-10553 0.19 0.14 0.61
EEEV-11424 0.29 0.43 1.58
WEEV-8304 0.64 0.85 3.19
VEEV-50 1.02 2.03 7.11
CHIKV-7427 0.23 0.37 1.34
MAL-1207 0.50 0.20 1.10
PF1-1187 0.10 0.14 0.52
PF2-1182 0.32 0.20 0.92
PV-1173 0.25 0.11 0.58
PM-1181 0.20 0.14 0.62
PO-1152 0.20 0.14 0.62
BM-113 0.43 0.42 1.69
WB-53 0.20 0.14 0.62
  1. Abbreviation: SD standard deviation
  2. aCut-off value of a probe (grey value) = average signal values of to detect other pathogens (except the particular pathogen detected by this probe) + 3 × standard deviation. A probe signal was considered positive when the value was greater than the cut-off value of a given probe