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Table 5 Cut-off values of the capture probes

From: Ultra-sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito-borne viruses and parasites

Probe

Average of negative sample (grey value)

SD

(grey value)

Cut-off valuea

(grey value)

DEN-10731

0.50

0.67

2.51

DEN1-X10830

0.18

0.33

1.17

DEN2-X10831

0.85

1.55

5.50

DEN3-X10829

0.38

0.29

1.25

DEN4-X10714

0.15

0.22

0.81

JEV-10726

0.42

0.30

1.32

YFV-10679

1.90

3.80

13.30

WNV-10768

0.30

0.39

1.47

SLE-10553

0.19

0.14

0.61

EEEV-11424

0.29

0.43

1.58

WEEV-8304

0.64

0.85

3.19

VEEV-50

1.02

2.03

7.11

CHIKV-7427

0.23

0.37

1.34

MAL-1207

0.50

0.20

1.10

PF1-1187

0.10

0.14

0.52

PF2-1182

0.32

0.20

0.92

PV-1173

0.25

0.11

0.58

PM-1181

0.20

0.14

0.62

PO-1152

0.20

0.14

0.62

BM-113

0.43

0.42

1.69

WB-53

0.20

0.14

0.62

  1. Abbreviation: SD standard deviation
  2. aCut-off value of a probe (grey value) = average signal values of to detect other pathogens (except the particular pathogen detected by this probe) + 3 × standard deviation. A probe signal was considered positive when the value was greater than the cut-off value of a given probe