Skip to main content
Fig. 4 | Parasites & Vectors

Fig. 4

From: Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein

Fig. 4

Expression of rCSUI_005805. Lane M: molecular weight marker. a Analysis of expressed recombinant protein by SDS-PAGE: uninduced culture (Lane 1); induced culture with 1 mM IPTG for 0.5 h (Lane 2) and 1 h (Lane 3), arrows indicate target protein bands of ~42 kDa. b SDS-PAGE analysis of NiNTA spin column purified rCSUI_005805: elution 1 (Lane 1); elution 2 (Lane 2), arrows indicate faint protein bands of ~25 kDa; naïve merozoite lysate (Lane 3). c Batch purified rCSUI_005805 using NiNTA agarose: stepwise elution (Lanes 1–5), arrows indicate faint protein bands of ~42 kDa. d Immunoblot of batch purified rCSUI_005805 probed with anti-His HRP conjugate (Lane 1)

Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us