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Fig. 5 | Parasites & Vectors

Fig. 5

From: Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein

Fig. 5

Immunoblot analyses of rCSUI_005805 and naïve merozoite lysate. The expressed recombinant protein and crude merozoite lysate were separated by SDS-PAGE and transferred to NC membranes. Lane M: Molecular weight marker (ordinate values describe band sizes in kDa); a Immunoblot of rCSUI_005805 probed with porcine anti-C. suis polyclonal sera (Lane 1) and with porcine pre-colostral sera (Lane 2). b Immunoblot of rCSUI_005805 probed with negative chicken serum (Lane 1) and chicken anti-rCSUI_005805 polyclonal sera (Lane 2). c Immunoblot of insoluble (Lanes 1–3) and soluble fractions (Lanes 4–6) of naïve merozoite lysate probed with porcine anti-C. suis polyclonal sera (Lanes 1 and 4), chicken anti-rCSUI_005805 polyclonal sera (Lanes 3 and 6) and negative chicken sera (Lanes 2 and 5), respectively. d Immunoblot of naïve merozoite lysate (Lanes 1 and 2) and rCSUI_005805 (Lane 3) probed with field sera from chickens vaccinated with HIPRACOX®

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Parasites & Vectors

ISSN: 1756-3305

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