Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein

Table 1 Oligonucleotide primers used for qPCR to determine stage-dependent transcription of CSUI_005805

Gene name	Forward primer sequence 5′-3′	Reverse primer sequence 5′- 3′	Amplicon length (bp)
GAPDH	ATTGGTCGTCTCGTGTTCCG	GATCGCACTTGGCTCCTTCT	216
ACT	CTTGCTGGCCGTGATTTGAC	ATATTGCCGTCCGGAAGCTC	203
18S rRNA	GCCAGTAGTCATATGCTTGTC	CTAATAAACACTGCCCTTCCTG	195
LSU rRNA	TGATTCCGAAGAGTGAGGC	CCAGGCGAAACTATAAAGCAG	195
CSUI_005805	CCTGAAAGTCGCCTGTCCAT	GACGCGTCAGCCGTTATAGT	224

GAPDH Glyceraldehyde-3-phosphate, ACT Actin, 18S rRNA 18S ribosomal RNA, LSU rRNA Large subunit ribosomal RNA

ISSN: 1756-3305