Groups | Groups (No. of animals) | 1st (0w) | 2nd (2w) | 3rd (4w) | 4th (6w) |
|---|
High dosage (μg) | ACT-F (14) | 100 | 100 | 100 | 100 |
ACT-T (15) | 100 | 100 | 100 | 100 |
Low dosage (μg) | ACT-F (13) | 40 | 20 | 20 | 20 |
ACT-T (18) | 40 | 20 | 20 | 20 |
Whole-cell antigen (13)a
| 40 | 20 | 20 | 20 |
Control (μl) | FA-sham (14)b
| 200 | 200 | 200 | 200 |
Unimmunized (20)c
| 200 | 200 | 200 | 200 |
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a
T. vaginalis was made into whole-cell antigen by sonication and alternate freeze/thaw treatment (in liquid nitrogen and 37 °C). The whole-cell antigen was quantified by using a Bicinchoninic Acid Kit (BCA). Before vaccination, it was emulsified in Freund’s adjuvant
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bEach mouse, 100 μl PBS emulsified in 100 μl completed Freund’s adjuvant for the 1st immunization, in incomplete Freund’s adjuvant for 2nd, 3rd and 4th immunizations
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cEach mouse was injected with only 200 μl sterile PBS using the same procedure in other groups
