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Table 4 Specimens of Aedes japonicus (CH) (n = 24) examined microscopically for larval stages of Dirofilaria immitis

From: Development of Dirofilaria immitis and Dirofilaria repens in Aedes japonicus and Aedes geniculatus

 

Mosquitoes

Number of larval stages per individual positive mosquito

 

Dpi

Total no.

No. positive

mf

L1

L2

L3

Location (total no. in all dissected mosquitoes)c

1

3

2

5, 4

–

–

–

 

3

6

6

1, 1, 1, 1

5, 4, 2, 1

–

–

4

3

1b

–

6

–

–

7

3

2

–

7

5, 3

1

In Malpighian tubules, L1 melanized

10

3

2

–

–

–

1, 6

In Malpighian tubules (7)

14

3a

3

–

–

1

1, 3

Head (1), Malpighian tubules (2)

21

3

3

–

–

1

1, 4, 9

Proboscis (7), head (1), thorax (5)

  1. Abbreviations: dpi day post-inoculation, mf microfilariae, L1 first-stage larva, L2 second-stage larva, L3 third-stage larva (infectious stage)
  2. aProboscis of one mosquito was lost during the dissection process
  3. bAdditional two positive in PCR only
  4. cData given only when localisation was clearly assignable after dissection