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Table 8 Specimens of laboratory colony Aedes aegypti IPNC (n = 22) examined microscopically for larval stages of Dirofilaria immitis

From: Development of Dirofilaria immitis and Dirofilaria repens in Aedes japonicus and Aedes geniculatus

   

Number of larval stages per individual positive mosquitoc

Dpi

Total no. of individuals

No. of positive individuals

mf

L1

L2

L3

1

9

5a

1, 1, 5, 7, 10

–

–

–

2

1

1

–

1

–

–

3

4

1b

1

–

–

–

5

2

1

–

3

–

–

7

2

0a

–

–

–

–

9

1

0b

–

–

–

–

12

1

0b

–

–

–

–

14

1

0b

–

–

–

–

16

1

0b

–

–

–

–

  1. Abbreviations: dpi day post-inoculation, mf microfilariae, L1 first-stage larva, L2 second-stage larva, L3 third-stage larva (infectious stage)
  2. aTwo additional positive in PCR
  3. bOne additional positive in PCR only
  4. cLocalisation of developmental stages was not clearly assignable after dissection