Parasites & Vectors

Table 1 Optimum calibration of the SBP4 MI-ELISA using a reference serum panel

From: A novel modified-indirect ELISA based on spherical body protein 4 for detecting antibody during acute and long-term infections with diverse Babesia bovis strains

Sample, dilution^a	Mean OD	S/N ratio	Result^b
Positive serum C167, 1/64	0.679	7.5	+
Positive serum C167, 1/128	0.394	4.3	+
Positive serum C167, 1/256	0.278	3.1	+
Positive serum C167, 1/512	0.187	2.1	–
Negative serum #37	0.123	1.3	–
Negative serum #312	0.149	1.6	–

Abbreviations: OD optical density, S OD of test sample, N OD of negative control
^aPositive serum was diluted with negative serum
^bSample considered positive if the S/N ratio was ≥ 3

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ISSN: 1756-3305

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