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Table 3 Diagnostic sensitivities of the SBP4 MI-ELISA and RAP-1 cELISA for B. bovis. All sera were positive based on immunofluorescent antibody assay. The cutoff for a SBP4 MI-ELISA positive result was a ≥ 3 S/N ratio, the cutoff for RAP-1 cELISA was ≥ 21% inhibition, and the cutoff for IFA was ≥ 1+ fluorescence

From: A novel modified-indirect ELISA based on spherical body protein 4 for detecting antibody during acute and long-term infections with diverse Babesia bovis strains

Sample origin (number)

SBP4 MI-ELISA

RAP-1 cELISA

Sera collected from six experimentally-infected calves (402)

100% (402/402)

84.6% (340/402)

Sera collected from herds vaccinated and/or challenged with virulent W strain in Australia (228)

95.2% (217/228)

86.0% (196/228)

Sera collected from B. bovis-endemic regions of Mexico (598)

98.7% (590/598)

60.0% (359/598)