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Fig. 10 | Parasites & Vectors

Fig. 10

From: Heat shock proteins 70 and 90 from Clonorchis sinensis induce Th1 response and stimulate antibody production

Fig. 10

rCsHSPs stimulate the production of anti-CsProR peptide antibodies via peptide interaction. Freund’s adjuvant (FA) or rCsHSPs (30 μg/ml) was incubated with CsProR peptide (100 μg/ml) for 1 h at room temperature. C57BL/6 mice were immunized with peptide immersed in FA or rCsHSP/peptide complexes. a T cells and sera were obtained from immunized mice on day 7 after final immunization. T cells were treated with PBS or 10 μg/ml CsProR peptide for 72 h, and proliferation was then evaluated by WST-1 assay. Anti-CsProR antibodies (b) and their isotypes (c) detected in immunized mice serum by ELISA. The results are shown as the mean ± standard error (SEM). *P < 0.05, **P < 0.01, ***P < 0.001 compared to PBS-immunized mice group

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