Fig. 4

rCsHSPs increase production of intracellular IL-12 in CD11c⁺ mBMDCs. mBMDCs were stimulated with rCsHSP70 and rCsHSP90 for 18 h, then labeled with FITC-conjugated CD11c antibody before permeabilization. Intracellular cytokines were labeled with PE-conjugated antibodies against mouse IL-12, IL-10, and IFN-γ. The number of cytokine-producing cells was determined by flow cytometry