Fig. 6

mBMDCs matured by stimulation with rCsHSPs show increased T cell proliferation and T cell secretion of IL-2 and IFN-γ. mBMDCs were incubated overnight with rCsHSP70, rCsHSP90, or LPS as indicated. mBMDCs were then washed and co-cultured with CFSE-labeled T cells. a T cell proliferation was evaluated after 4, 5 and 6 days by flow cytometry. X- and y-axes indicates fluorescence intensity for CFSE and number of cells, respectively. b IL-2 secretion was quantified after 16 h; IL-4 and IFN-γ levels were measured after 5 days by ELISA. The results are shown as the mean ± standard error (SEM) and are representative of three independent experiments. *P < 0.05, **P < 0.01 compared to PBS-treated group