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Fig. 7 | Parasites & Vectors

Fig. 7

From: Heat shock proteins 70 and 90 from Clonorchis sinensis induce Th1 response and stimulate antibody production

Fig. 7

Cytokines secreted from splenocytes of PBS or rCsHSPs-immunized mice. Mice were immunized on day 0 with PBS or 100 μg rCsHSPs per mouse emulsified in complete Freund’s adjuvant followed by two booster injections with 100 μg rCsHSPs per mouse emulsified in incomplete Freund’s adjuvant on day 14th and 28th days. After the 7th day of the last immunization, the splenocytes were isolated from mice and were cultured either in the absence or in the presence of 5 μg/ml rCsHSP70 (a), rCsHSP90 (b) for 72 h. The results are shown as the mean ± standard error (SEM) and are representative of three independent experiments. **P < 0.01, ***P < 0.001

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