Fig. 1From: Identification of immune protective genes of Eimeria maxima through cDNA expression library screeningConstruction procedure of E. maxima cDNA expression library. a Sporulated oocyst of E. maxima. Scale-bar: 10 μm. b Quality determination of the isolated total RNA and mRNA of E. maxima by denaturing agarose gel electrophoresis. c Titer and insert size evaluation of the entry library using plating assay and PCR assay. d Titer and insert size evaluation of the cDNA expression library using plating assay and PCR assay. e Amplification of 7 known genes from the cDNA expression libraryBack to article page