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Table 5 Characterizations of the six protective clones screened from the cDNA expression library of E. maxima

From: Identification of immune protective genes of Eimeria maxima through cDNA expression library screening

Clones

Designated name

Insert size (bp)

ORF (bp)

Predicted molecular mass (kDa)

Isoelectric point

Predicted T cell epitope motifs

Regions with high antigenic index

GenBank accession number

8–2–2–2

EmHP–1

1162

957

35.49

5.05

4–13, 84–88, 96–99, 129–132, 151–154, 175–178, 197–201, 240–243, 250–260, 271–278, 281–284

10–33, 38–48, 69–145, 149–167, 173–217, 229–243

KR868754.1

8–2–2–5

EmSAG

1076

708

24.73

4.81

22–25, 39–42, 45–48, 63–66, 79–82, 106–109, 122–126, 135–138, 160–163, 172–179, 188–192, 200–203, 214–217

34–42, 45–50, 53–66, 68–88, 94–101, 115–124, 127–136, 144–151, 156–175

CDJ60815.1

8–2–8–1

EmRP

1004

774

28.34

8.27

8–11, 19–22, 60–64, 66–70, 90–97, 150–153, 195–198, 222–228, 245–249

1–10, 14–23, 27–35, 54–62, 105–118, 165–175, 212–220, 226–234

KR815509

8–2–8–2

EmHP–2

1261

753

27.91

5.01

29–32, 114–118, 133–139, 149–155, 160–163, 174–177, 192–195, 198–201, 225–232

11–27, 14–23, 32–43, 71–124, 135–152, 166–190, 243–2247, 226–234

KR868755.1

8–2–8–3

EmCKRS

1150

930

32.97

4.33

2–5, 7–10, 23–27, 59–63, 76–79, 139–142, 170–174, 208–211, 219–222, 229–233, 241–244, 247–250, 268–271, 275–279, 285–289

1–17, 24–42, 55–80, 94–109, 118–221, 232–258, 270–284, 292–309

CDJ61187.1

8–2–8–6

EmJS–1

1233

603

22.26

8.33

73–76, 78–81, 125–128, 132–136, 157–160, 163–166, 179–182, 192–195

54–64, 67–90, 110–118, 123–132, 137–145, 160–173, 180–200, 292–309

KR868753.1