Fig. 3
From: Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti
SINV infection induces expression of molecules from Toll, IMD and Jak/STAT pathways. Gene expression of the Toll, IMD and Jak/STAT pathways were evaluated by qPCR in three different tissues: carcass, midgut and ovaries. Tissues were dissected for RNA extraction 4 days post-infection. a Carcass. b Midgut. c Ovary. Relative expression was calculated using the ΔΔCt method comparing each infected tissue with the respective non-infected one, represented as 1 (blank bars). We performed Student’s t-test for statistical analysis between tissues infected and non-infected. RP49 was used as a reference gene