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Table 1 Primer designation, sequences and annealing temperatures of the conducted PCRs used for the detection of Bartonella spp. from Ixodes ricinus ticks

From: Molecular detection of Bartonella henselae in 11 Ixodes ricinus ticks extracted from a single cat

Target

Primer designation

Sequence (5′–3′)

Length (bp)

Annealing temperature (°C)

Reference

Bartonella spp. 16S rDNA (first round)

A-proteo primer

AGAGTTTGATC(AC)TGGCTCAGA

1,210

62 °C (1 min)

[19]

r-Alpha-sh primer

GTAGCACGTGTGTAGCCCA

Bartonella spp. 16S rDNA (nested PCR)

Bart

CACTCTTTTAGAGTGAGCGGCAA

990

65 °C (1 min)

[19]

r-BH

CCCCCTAGAGTGCCCAACCA

Bartonella 16S-23S ITS

325 s

CTTCAGATGATGATCCCAAGCCTTCTGGCG

489

68 °C (15 s)

[21]

1100as

GAACCGACGACCCCCTGCTTGCAAAGCA

Bad A head region

badAf8

TCGAATCTTGCGCTTACAGGAGC

325

59 °C (30 s)

Present study

BadA_head_reverse

CACCGTCAGTCGACTTCCCT

Vir B7 of the VirB/D4-type-IV-secretion-system

VirB7_for

GCTGGAAAACGAAAAAGCAA

103

55 °C (30 s)

Present study

VirB7_rev

ACGCGCAATCTCCATAGTGT