From: Molecular detection of Bartonella henselae in 11 Ixodes ricinus ticks extracted from a single cat
Target | Primer designation | Sequence (5′–3′) | Length (bp) | Annealing temperature (°C) | Reference |
---|---|---|---|---|---|
Bartonella spp. 16S rDNA (first round) | A-proteo primer | AGAGTTTGATC(AC)TGGCTCAGA | 1,210 | 62 °C (1 min) | [19] |
r-Alpha-sh primer | GTAGCACGTGTGTAGCCCA | ||||
Bartonella spp. 16S rDNA (nested PCR) | Bart | CACTCTTTTAGAGTGAGCGGCAA | 990 | 65 °C (1 min) | [19] |
r-BH | CCCCCTAGAGTGCCCAACCA | ||||
Bartonella 16S-23S ITS | 325 s | CTTCAGATGATGATCCCAAGCCTTCTGGCG | 489 | 68 °C (15 s) | [21] |
1100as | GAACCGACGACCCCCTGCTTGCAAAGCA | ||||
Bad A head region | badAf8 | TCGAATCTTGCGCTTACAGGAGC | 325 | 59 °C (30 s) | Present study |
BadA_head_reverse | CACCGTCAGTCGACTTCCCT | ||||
Vir B7 of the VirB/D4-type-IV-secretion-system | VirB7_for | GCTGGAAAACGAAAAAGCAA | 103 | 55 °C (30 s) | Present study |
VirB7_rev | ACGCGCAATCTCCATAGTGT |