Table 1 Primer sequences for Babesia genus and species-specific PCRs
Primer name | Gene target | Sequence (5′-3′) |
|---|---|---|
B-lsu-F | Babesia lsu5-lsu4 | ACCTGTCAARTTCCTTCACTAAMTT |
B-lsu-R2 | Babesia lsu5-lsu4 | TCTTAACCCAACTCACGTACCA |
Bmic-F | B. microti-like lsu5-lsu4 | TTGCGATAGTAATAGATTTACTGC |
Bcommon-F | Babesia 18S rRNA | GCATTTGCGATGGACCATTCAAG |
Bcommon-R | Babesia 18S rRNA | CCTGTATTGTTATTTCTTGTCACTACCTC |
BMIC18-F | B. microti-like 18S rRNA | CTGCTTTATCATTAATTTCGCTTCCGAACG |
BCV-F | B. vogeli 18S rRNA | GTTCGAGTTTGCCATTCGTT |
BCC-F | B. canis 18S rRNA | TTGCGTTGACGGTTTGACC |
BCO-F | B. coco 18S rRNA | CCTTTTCTTTGCTTTGTCGC |
BGNC-F | B. gibsoni 18S rRNA | ACTCGGCTACTTGCCTTGTC |
BAB722-R | Babesia 18S rRNA | ATGCCCCCAACCGTTCCTATTA |
BCV-cox1-F4 | B. vogeli cox1 | TGCTATGAGTGGCGCAAATTTTG |
BCV-cox1-R | B. vogeli cox1 | CCATACAGTAGGTATCAATCTATCT |
BCC-cox1-F2 | B. canis cox1 | GTGCAATGAGTGGAGCAAATTTCA |
BCC-cox1-R | B. canis cox1 | CCATACAGTTGGTATTAATCTATCC |
BCO-cox1-F2 | B. coco cox1 | TTGTAACTTCTGTTTTACTTATGGTG |
BCO-cox1-R2 | B. coco cox1 | AAAATAAGAATATAAACCTCAGGATGT |
BG-cox1-F | B. gibsoni cox1 | CTTCAGCCAATAGCTTTCTGTTTG |
BG-cox1-R | B. gibsoni cox1 | CCTGAGGCAAGTAAACCAAATAT |