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Table 3 The efficiency and analytical sensitivity for the Babesia LSU qPCR. The efficiency and analytical sensitivity were determined for the Babesia LSU qPCR using plasmids as template DNA run in 20 intra-assay replicates at 3 copies/well and 5 copies/well. Assays were run with all 3 primers (Bmic-F, B-lsu-F and B-lsu-R2) for both plasmids and each corresponding 2 primer reaction: Bmic-F with B-lsu-R2 for B. microti-like plasmid (pBMIC-LSU) and B-lsu-F with B-lsu-R2 for B. vogeli plasmid (pBCV-LSU)

From: Improved molecular detection of Babesia infections in animals using a novel quantitative real-time PCR diagnostic assay targeting mitochondrial DNA

Primers Plasmid template Eff (%) R 2 3 c/well (%) Cq (range) 5 c/well (%) Cq (range)
2 primers pBMIC-LSU 100 0.99 90 34.1–37.2 100 33.5–37.5
3 primers pBMIC-LSU 95 0.99 70 33.6–37.3 95 34.2–37.8
2 primers pBCV-LSU 94 0.99 60 37.1–39.9 100 33.3–36.7
3 primers pBCV-LSU 92 0.99 60 36.8–39.2 100 33–34.2
  1. Abbreviations: Eff efficiency, C copies