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Table 5 Positive LSU qPCR results were compared with 18S qPCR results from a prospective analysis performed on canine diagnostic samples naturally infected with Babesia spp.

From: Improved molecular detection of Babesia infections in animals using a novel quantitative real-time PCR diagnostic assay targeting mitochondrial DNA

Total samples (n = 394)

18S

LSU

B. canis (+)

2

2

B. vogeli (+)

2

2

B. coco (+)

0

2

B. gibsoni (+)

7

12

B. microti-like (+)

0

1

% Positive (95% CI)

2.8 (1.1–4.4%)

4.8 (3.1–7.5%)

  1. (+) = Cq value obtained, T m value was correct