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Table 1 Salivary proteins of female R. microplus ticks recognized by sera from naïve and/or two-times infested genetically tick-susceptible and/or tick-resistant bovines

From: Immune recognition of salivary proteins from the cattle tick Rhipicephalus microplus differs according to the genotype of the bovine host

Spot No. in figure CDS in sialotranscriptomea Annotation and putative function of protein MW Kdab Source of pooled reactive serac
34 20757 14-3-3 CG17870-PA, isoform A isoform 2 27 NR IR
33 20757 14-3-3 CG17870-PA, isoform A isoform 2 27 NR IR
20 18420 actin 42 IR
21 18420 actin 42 IR
19 18420 actin 42 IR IS
22 18420 actin 42 IR NS
1 109972 anticoagulant protein rhipilin-1 17 NR IR NS IS
37 31546 apolipophorin 89 NR IR
18 31546 apolipophorin 89 NR IR
22 7197 beta tubulin 53 IR NS
22 22306 beta tubulin 50 IR NS
22 22310 beta tubulin 35 IR NS
29 14491 cathepsin D2 42 NS IS NR IR
20 39114 cathepsin L-like cysteine proteinase B 38 IR
21 39114 cathepsin L-like cysteine proteinase B 38 IR
19 39114 cathepsin L-like cysteine proteinase B 38 IR IS
1 50205 CBF1-interacting corepressor 30 NR IR NS IS
40 30215 chaperonin subunit 65 IR
9 111414 CRISP3-cysteine-rich secretory protein 46 IR NS
30 25106 enolase 43 IR
24 67998 ENSANGP00000022132 25 NR IR
22 4388 F0F1-type ATP synthase, beta subunit 59 IR NS
25 111630 glutathione S-transferase 31 NR
24 111630 glutathione S-transferase 31 NR IR
38 21956 heat shock protein 71 NS
37 4142 heavy-chain filboin 44 NR IR
14 71787 hypothetical protein 22 NR IR IS
1 110513 hypothetical protein 3 NR IR NS IS
38 121267 hypothetical protein BRAFLDRAFT_287019 45 NS
20 122308 longipain cystein protease 33 IR
21 122308 longipain cystein protease 33 IR
19 122308 longipain cystein protease 33 IR IS
13 113489 lospin 8 type serpin 43 IR IS
12 113489 lospin 8 type serpin 43 NR IR IS
11 113489 lospin 8 type serpin 43 NR IR NS IS
6 113489 lospin 8 type serpin 43 NR IR NS IS
7 113489 lospin 8 type serpin 43 NR IR NS IS
10 113489 lospin 8 type serpin 43 IR
8 113489 lospin 8 type serpin 43 IR IS
5 113489 lospin 8 type serpin 43 NR IR IS
11 171727 lysosomal acid phosphatase 26 NR IR NS IS
40 9166 protein disulfite isomerase-2 39 IR
40 10534 protein disulfite isomerase-2 39 IR
12 39744 putative chitinase 45 NR IR IS
15 128399 putative salivary secreted protein 36 IR IS
24 83247 putative salivary secreted protein 25 NR IR
14 77570 putative salivary secreted protein 25 NR IR IS
14 85307 putative salivary secreted protein 27 NR IR IS
35 108605 putative secreted protein 24 IR
35 171393 putative secreted protein 18 IR
32 171393 putative secreted protein 18 NR IR
33 171393 putative secreted protein 18 NR IR
34 171393 putative secreted protein 18 NR IR
30 174664 putative secreted protein (histamine-binding?) 21 IR
31 174664 putative secreted protein (histamine-binding?) 21 NR IR
32 174664 putative secreted protein (histamine-binding?) 21 NR IR
33 174664 putative secreted protein (histamine-binding?) 21 NR IR
34 174664 putative secreted protein (histamine-binding?) 21 NR IR
31 178127 putative secreted protein (histamine-binding?) 21 NR IR
33 178127 putative secreted protein (histamine-binding?) 21 NR IR
34 178127 putative secreted protein (histamine-binding?) 21 NR IR
35 178127 putative secreted protein (histamine-binding?) 21 IR
14 54776 putative thyropin precursor 23 NR IR IS
17 113785 salivary lipid interacting protein 20 NR IR
36 113785 salivary lipid interacting protein 20 NR IR
16 113785 salivary lipid interacting protein 20 NR IR
8 38904 secreted protein 38 IR IS
31 164102 secreted protein 23 NR IR
31 164103 secreted protein 23 NR IR
33 164103 secreted protein 23 NR IR
7 38904 secreted protein 38 NR IR NS IS
16 128399 secreted protein 36 NR IR
17 128399 secreted protein 36 NR IR
26 8103 secreted salivary gland peptide 25 IS IR
25 8103 secreted salivary gland peptide 25 NR
24 8103 secreted salivary gland peptide 25 NR IR
27 8103 secreted salivary gland peptide 25 NR IR NS
24 26121 selenium-dependent salivary glutathione peroxidase 18 NR IR
20 106322 serine proteinase inhibitor serpin-3 43 IR
21 106322 serine proteinase inhibitor serpin-3 43 IR
19 106322 serine proteinase inhibitor serpin-3 43 IR IS
30 106322 serine proteinase inhibitor serpin-3 43 IR
20 106321 serine proteinase inhibitor serpin-3 43 IR
19 106321 serine proteinase inhibitor serpin-3 43 IR IS
12 6955 serpin-2 precursor 36 NR IR IS
28 13343 translation elongation factor EF-1 alpha-Tu 51 NS IS
29 13343 translation elongation factor EF-1 alpha-Tu 51 NS IS NR IR
31 32190 uncharacterized protein 17 NR IR
37 43507 vitellogenin-1 158 NR IR
18 43508 vitellogenin-1 97 NR IR
18 43509 vitellogenin-1 182 NR IR
  1. Note: The table is formatted according to annotation of putative function of protein, but not by spot number recovered from gel. In some instances there are more than one protein identified in the spot collected
  2. aCDS: coding sequences generated by the sialotranscriptome of R. microplus and deposited at GenBank - NCBI (BioProject ID PRJNA329522)
  3. bMolecular weight furnished by Scaffold software
  4. c Abbreviations for sources of sera and samples: NR, pooled sera collected from four tick-naïve, genetically resistant Nelore; IR, pooled sera from four genetically resistant, twice-infested Nelore bovines at the end of the infestation; NS, pooled sera collected from four tick-naïve, genetically susceptible Holstein bovines; IS, pooled sera from four genetically susceptible, twice-infested Holstein bovines at the end of the infestation
  5. Salivary proteins were obtained from saliva of female R. microplus ticks feeding on genetically susceptible hosts. Sera employed were obtained from naïve and/or two-times infested genetically tick-susceptible and/or tick -resistant bovines. Criteria employed to identify each sequence: minimum of one peptide presenting with 90% probability of being the protein and 90% probability of being the peptide