Fig. 6From: Secreted phospholipase A2 of Clonorchis sinensis activates hepatic stellate cells through a pathway involving JNK signallingCollagen I produced by HSCs are increased after incubated with MBP-CssPLA2. The effect of MBP-CssPLA2 on activation of hepatic stellate cells and production of collagen I was evaluated by ELISA. After 36Â h or 48Â h, the supernatant of cells was detected by ELISA. Collagen I rabbit antibody (1:2000 dilutions) was used as the first antibody and HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody (1:10,000 dilution). a Supernatant of cell culture after incubation for 36Â h; b supernatant of cell culture after incubation for 48Â h. Unpaired t-test was applied for statistical analysisBack to article page