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Fig. 6 | Parasites & Vectors

Fig. 6

From: Secreted phospholipase A2 of Clonorchis sinensis activates hepatic stellate cells through a pathway involving JNK signalling

Fig. 6

Collagen I produced by HSCs are increased after incubated with MBP-CssPLA2. The effect of MBP-CssPLA2 on activation of hepatic stellate cells and production of collagen I was evaluated by ELISA. After 36 h or 48 h, the supernatant of cells was detected by ELISA. Collagen I rabbit antibody (1:2000 dilutions) was used as the first antibody and HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody (1:10,000 dilution). a Supernatant of cell culture after incubation for 36 h; b supernatant of cell culture after incubation for 48 h. Unpaired t-test was applied for statistical analysis

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