Fig. 8

HSCs are activated after incubated with either MBP-CssPLA2 or the mutant. Quantitative RT-PCR analysis of activation markers of LX-2 was performed. The mRNA of α-SMA and collagen III were detected, respectively. The transcription levels of α -SMA and collagen III were analyzed by means of the 2^-△△Ct ratio, with human β-actin serving as the internal standard. The transcription level of α-SMA and collagen III in LX-2 cells incubated with 25 μg/ml mutant and 25 μg/ml MBP-CssPLA2 was similar, with no statistical significance, while that of LX-2 cells incubated with both 25 μg/ml MBP-CssPLA2 and 5 μM JNK inhibitor (SP600125) was lower than that of cells incubated with 25 μg/ml MBP-CssPLA2. Unpaired t-test was applied to compare the difference between experimental group and control group. a The relative levels of mRNA for α-SMA of three groups of LX-2 cells incubated with 25 μg/ml mutant, 25 μg/ml MBP-CssPLA2 and both 25 μg/ml MBP-CssPLA2 and 5 μM JNK inhibitor (SP600125), respectively (t ₍₄₎ = 3.905, P = 0.0175). b Relative levels of mRNA for collagen III of three groups of LX-2 cells incubated with 25 μg/ml mutant, 25 μg/ml MBP-CssPLA2 and both 25 μg/ml MBP-CssPLA2 and 5 μM JNK inhibitor (SP600125), respectively (t ₍₄₎ = 4.095, P = 0.0149)