The contribution of dietary restriction to extended longevity in the malaria vector Anopheles coluzzii

Parasites & Vectors

Table 3 The effects of temperature, RH, and nutritional regime, with interactions on longevity: Model 1, by treatment (= exposure) Cox regression, with varying conditions of temperature and relative humidity. Model 2, with time-dependent diet-regime variables and varying conditions of sugar or blood consumption

Parameter^a	Model 1 (Treatment)^b -2LL = 15819.69, AIC = 15,819.69		Model 2 (Time-dependent)^c -2LL = 14649.21, AIC = 14661.21		Estimates of parameter effects^d
	Model 1 (Treatment)^b -2LL = 15819.69, AIC = 15,819.69		Model 2 (Time-dependent)^c -2LL = 14649.21, AIC = 14661.21		Model 1		Model 2
	Estimate (χ ²)	P-value^e	Estimate (χ ²)	P-value^e	Conditions	Hazard ratio (95% CI)	Conditions	Hazard Ratio (95% CI)
Temperature	0.027 (0.26)	0.610	-0.095 (3.22)	0.073	∆5 °C & 50% RH	1.28 (1.06–1.53)
Temperature	0.027 (0.26)	0.610	-0.095 (3.22)	0.073	∆5 °C & 85% RH	2.12 (1.74–2.58)
RH	-0.054 (8.07)	0.005	-0.078 (16.34)	< 0.0001	∆10% RH & 22 °C	0.86 (0.82–0.91)
RH	-0.054 (8.07)	0.005	-0.078 (16.34)	< 0.0001	∆10% RH & 27 °C	1.00 (0.95–1.04)
Sugar	-4.714 (135.35)	< 0.0001	-0.122 (86.45)	< 0.0001	BM 1/10 d, Sug 0.14	0.66 (0.47–0.93)	1 BM/2 Sug	0.35 (0.32–0.38)
					BM 1/10 d, Sug 0.33	1.35 (0.97–1.88)	1 BM/14 Sug	0.45 (0.41–0.49)
					BM 1/10 d, Sug 1	17.14 (9.57–30.69)	1 BM/50 Sug	0.94 (0.69–1.29)
Blood	-9.516 (23.88)	< 0.0001	-1.089 (616.51)	< 0.0001	Sug 1/7 d, BM 0	0.51 (0.46–0.57)	1 Sug/BL 0	0.89 (0.86–0.91)
					Sug 1/7 d, BM 0.05	0.67 (0.62–0.72)	1 Sug/BL 4	0.96 (0.95–0.97)
					Sug 1/7 d, BM 0.15	1.15 (1.10–1.21)	1 Sug/BL 9	1.07 (1.02–1.11)
Temp x RH	0.002 (5.84)	0.016	0.003 (13.97)	0.0002
Sug*BL	37.928 (120.07)	< 0.0001	0.021 (31.94)	< 0.0001
Time*BL	-0.379 (22.45)	< 0.0001	-0.380 (22.45)	< 0.0001

Bold P-values indicate statistical significance
^aAll parameters had 1 df
^bBased on allocation to treatment (= cage)
^cBased on allocation by day of death
^dEstimates based on condition ranges relevant to laboratory and field conditions
^eSignificant P-values shown in bold-face
Abbreviations: BM blood meal (1/10 = once every 10 days); Sug sugar (1/7 = once every 7 days); RH relative humidity