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Table 3 The effects of temperature, RH, and nutritional regime, with interactions on longevity: Model 1, by treatment (= exposure) Cox regression, with varying conditions of temperature and relative humidity. Model 2, with time-dependent diet-regime variables and varying conditions of sugar or blood consumption

From: The contribution of dietary restriction to extended longevity in the malaria vector Anopheles coluzzii

Parametera

Model 1 (Treatment)b

-2LL = 15819.69, AIC = 15,819.69

Model 2 (Time-dependent)c

-2LL = 14649.21, AIC = 14661.21

Estimates of parameter effectsd

Model 1

Model 2

Estimate (χ 2)

P-valuee

Estimate (χ 2)

P-valuee

Conditions

Hazard ratio (95% CI)

Conditions

Hazard Ratio (95% CI)

Temperature

0.027 (0.26)

0.610

-0.095 (3.22)

0.073

∆5 °C & 50% RH

1.28 (1.06–1.53)

 

∆5 °C & 85% RH

2.12 (1.74–2.58)

RH

-0.054 (8.07)

0.005

-0.078 (16.34)

< 0.0001

∆10% RH & 22 °C

0.86 (0.82–0.91)

∆10% RH & 27 °C

1.00 (0.95–1.04)

Sugar

-4.714 (135.35)

< 0.0001

-0.122 (86.45)

< 0.0001

BM 1/10 d, Sug 0.14

0.66 (0.47–0.93)

1 BM/2 Sug

0.35 (0.32–0.38)

BM 1/10 d, Sug 0.33

1.35 (0.97–1.88)

1 BM/14 Sug

0.45 (0.41–0.49)

BM 1/10 d, Sug 1

17.14 (9.57–30.69)

1 BM/50 Sug

0.94 (0.69–1.29)

Blood

-9.516 (23.88)

< 0.0001

-1.089 (616.51)

< 0.0001

Sug 1/7 d, BM 0

0.51 (0.46–0.57)

1 Sug/BL 0

0.89 (0.86–0.91)

Sug 1/7 d, BM 0.05

0.67 (0.62–0.72)

1 Sug/BL 4

0.96 (0.95–0.97)

Sug 1/7 d, BM 0.15

1.15 (1.10–1.21)

1 Sug/BL 9

1.07 (1.02–1.11)

Temp x RH

0.002 (5.84)

0.016

0.003 (13.97)

0.0002

 

Sug*BL

37.928 (120.07)

< 0.0001

0.021 (31.94)

< 0.0001

Time*BL

-0.379 (22.45)

< 0.0001

-0.380 (22.45)

< 0.0001

  1. Bold P-values indicate statistical significance
  2. aAll parameters had 1 df
  3. bBased on allocation to treatment (= cage)
  4. cBased on allocation by day of death
  5. dEstimates based on condition ranges relevant to laboratory and field conditions
  6. eSignificant P-values shown in bold-face
  7. Abbreviations: BM blood meal (1/10 = once every 10 days); Sug sugar (1/7 = once every 7 days); RH relative humidity