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Table 3 The effects of temperature, RH, and nutritional regime, with interactions on longevity: Model 1, by treatment (= exposure) Cox regression, with varying conditions of temperature and relative humidity. Model 2, with time-dependent diet-regime variables and varying conditions of sugar or blood consumption

From: The contribution of dietary restriction to extended longevity in the malaria vector Anopheles coluzzii

Parametera Model 1 (Treatment)b -2LL = 15819.69, AIC = 15,819.69 Model 2 (Time-dependent)c -2LL = 14649.21, AIC = 14661.21 Estimates of parameter effectsd
Model 1 Model 2
Estimate (χ 2) P-valuee Estimate (χ 2) P-valuee Conditions Hazard ratio (95% CI) Conditions Hazard Ratio (95% CI)
Temperature 0.027 (0.26) 0.610 -0.095 (3.22) 0.073 ∆5 °C & 50% RH 1.28 (1.06–1.53)  
∆5 °C & 85% RH 2.12 (1.74–2.58)
RH -0.054 (8.07) 0.005 -0.078 (16.34) < 0.0001 ∆10% RH & 22 °C 0.86 (0.82–0.91)
∆10% RH & 27 °C 1.00 (0.95–1.04)
Sugar -4.714 (135.35) < 0.0001 -0.122 (86.45) < 0.0001 BM 1/10 d, Sug 0.14 0.66 (0.47–0.93) 1 BM/2 Sug 0.35 (0.32–0.38)
BM 1/10 d, Sug 0.33 1.35 (0.97–1.88) 1 BM/14 Sug 0.45 (0.41–0.49)
BM 1/10 d, Sug 1 17.14 (9.57–30.69) 1 BM/50 Sug 0.94 (0.69–1.29)
Blood -9.516 (23.88) < 0.0001 -1.089 (616.51) < 0.0001 Sug 1/7 d, BM 0 0.51 (0.46–0.57) 1 Sug/BL 0 0.89 (0.86–0.91)
Sug 1/7 d, BM 0.05 0.67 (0.62–0.72) 1 Sug/BL 4 0.96 (0.95–0.97)
Sug 1/7 d, BM 0.15 1.15 (1.10–1.21) 1 Sug/BL 9 1.07 (1.02–1.11)
Temp x RH 0.002 (5.84) 0.016 0.003 (13.97) 0.0002  
Sug*BL 37.928 (120.07) < 0.0001 0.021 (31.94) < 0.0001
Time*BL -0.379 (22.45) < 0.0001 -0.380 (22.45) < 0.0001
  1. Bold P-values indicate statistical significance
  2. aAll parameters had 1 df
  3. bBased on allocation to treatment (= cage)
  4. cBased on allocation by day of death
  5. dEstimates based on condition ranges relevant to laboratory and field conditions
  6. eSignificant P-values shown in bold-face
  7. Abbreviations: BM blood meal (1/10 = once every 10 days); Sug sugar (1/7 = once every 7 days); RH relative humidity