Fig. 2
Nucleotide diversity in the IIS6 NaV segment of Aedes albopictus. ClustalW alignment for comparing the haplotypes found in this study, IIS6_Hap1 (KX281169) and IIS6_Hap2 (KX281170), with other sequences of Ae. albopictus available in GenBank from Brazil (FJ479615), Malaysia (KC152045) and Japan (AB827810). One haplotype of Ae. aegypti (FJ479611) was included for comparison, but the intron was neglected in order not to disturb the alignment. The sequence AB827810 does not cover the whole extension of this alignment. The translated amino acid sequence is represented by the letters in blue, over the first nucleotide of their corresponding codon. Nucleotides in uppercase letters correspond to the coding region (exons 20 and 21), and those in lower case refer to the intron with the numbering in the alignment inside brackets at the top of each block and underlined sequences referring to the primers positions. Invariable sites are indicated with dots, otherwise with the alternative nucleotide and gaps with (-). Squares indicate the codons where the 989, 1011 and 1016 kdr mutation sites are present in Ae. aegypti