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Fig. 3 | Parasites & Vectors

Fig. 3

From: First report of the Phe1534Cys kdr mutation in natural populations of Aedes albopictus from Brazil

Fig. 3

Nucleotide diversity in the IIIS6 NaV segment of Aedes albopictus. ClustalW alignment for comparing the haplotypes found in this study, 1534Phe (KX371864) and 1534Cys (KX371865), with other Ae. albopictus sequences available in GenBank: genomic DNA (AB827824), mRNA (KC152046 and AY663382) and one haplotype of Ae. aegypti (KF527415). The three sequences downloaded from GenBank do not cover the whole extension of this alignment. The translated amino acid sequence is represented by the letters in blue, over the first nucleotide of their corresponding codon. Nucleotides in uppercase letters correspond to the coding region (exons 30 and 31) and those in lower case refer to the intron, with the numbering in the alignment inside brackets at the top of each block and single underlined sequences referring to the primers positions. Double underlines indicate the annealing region for the AS-PCR primers. Invariable sites are indicated with dots, otherwise with the alternative nucleotide and gaps with (-). The 1534 kdr site is indicated with a square

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