Fig. 2
From: Identification and functional characterisation of a Schistosoma japonicum insulin-like peptide
Western blot analysis using anti-SjILP serum to probe recombinant proteins (SjILP, SmILP and human insulin) and total extracts from adult schistosomes. a The SDS-PAGE gel of purified and refolded recombinant proteins SjILP (Lane 1) and SmILP (Lane 2). Mouse anti-SjILP antibody was used to probe rSjILP (Lane 3), rSmILP (Lane 4); b Mouse anti-SjILP antibody was used to probe carcass protein (Lane 1) and tegument protein (Lane 2) extracted from adult S. japonicum, and SWAP extracted from adult S. mansoni (Lane 3); c Mouse anti-human insulin monoclonal antibody was used to probe recombinant human insulin (Lane 1) and rSjILP (Lane 2). Mouse anti-SjILP antibody was used to probe recombinant human insulin (Lane 3) and rSjILP (Lane 4). Lanes M1 and M2, PageRuler pre-stained protein ladder in a different size. This experiment was performed three times