Fig. 3
From: Identification and functional characterisation of a Schistosoma japonicum insulin-like peptide
Effect of stimulation with rSjILP or human insulin on extracellular signal-regulated kinase (Erk) in adult S. japonicum worms. a Anti-actin antibody (upper) and anti-phospho p44/42 MAPK (Erk) antibody (lower) were used to probe in Western blot analysis a protein extract of adult S. japonicum incubated for 30Â min with: rSjILP (Lane 1); both rSjILP and human insulin (Lane 2); human insulin (Lane 3); neither rSjILP nor insulin (Lane 4). b Signal intensities of bands recognised by anti-phospho p44/42 MAPK (Erk) antibody in panel A (lower), were measured using the Odyssey Classic Infrared Imager with a scan intensity setting of 5 and sensitivity of 5. Error bars represent the standard error of the mean (SEM). This experiment was performed twice