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Fig. 5 | Parasites & Vectors

Fig. 5

From: Identification and functional characterisation of a Schistosoma japonicum insulin-like peptide

Fig. 5

Binding affinity assays between SjILP/human insulin and SjLD1/2 using the Octet RED system. The real-time binding response between rSjILP and (a) rSjLD1, (b) rSjLD2 at different protein concentrations (ng/μl) was measured in seconds (s). The parameters of the binding response (nm) and the KD value (M) of the binding between SjILP and SjLD1/2 (7–112 ng/μl) are shown. c A comparison of the SjLD1 binding ability to SjILP and human insulin at concentrations of 65 and 130 ng/μl. d A comparison of the SjLD2 binding ability to SjILP and human insulin at concentrations 65 and 130 ng/μl. The coefficient of determination (R 2) for all the interactions was close to 1.0, indicating a good curve fit

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