Fig. 1

Gene structure of AsLac2, phylogenetic analysis of Laccase 1 (LAC1) and Laccase 2 (LAC2) proteins among representative insect species, and genome synteny of the neighborhood of Laccase 2. a Solid red boxes represent amino acid coding sequences and fold lines represent the introns. Solid black box represents the 3′UTR. Green square brackets (horizontal) represent the Cu-oxidase domains. b Maximum likelihood phylogenetic tree of Laccase 1 and Laccase 2 among different insects. Amino acid sequence information are as follow: Anopheles gambiae (LAC1: AGAP003738-PA; LAC2: AGAP006176-PB), Anopheles coluzzii (LAC1: ACOM034942-PA; LAC2: ACOM024569-PA), Anopheles arabiensis (LAC1: AARA010056-PA; LAC2: AARA007346-PA BAG70891), Anopheles funestus (LAC1: AFUN001510-PA; LAC2: AFUN004369-PA), Anopheles stephensi (LAC1: ASTE005343-PA; LAC2: ASTE006211-PA), Anopheles dirus (LAC1: ADIR002561-PA; LAC2: ADIR007990-PA), Anopheles darling (LAC1: ADAC007761-PA; LAC2: ADAC006306 -PA), Aedes aegypti (LAC1: AAEL007802 -PA; LAC2: AAEL007415-PA), Culex quinquefasciatus (LAC1: CPIJ012357-PA; LAC2: CPIJ010466-PA), Bombyx mori (LAC1: XP_012552135; LAC2: BAG70891), Manduca sexta (LAC1: AY135185; LAC2: AAN17507), Drosophila melanogaster (LAC1: NP_609287; LAC2: NP_724412), and Tribolium castaneum (LAC1: NP_001034514; LAC2: NP_001034487); c Genomic or scaffold synteny analysis of the neighborhood of Laccase 2 in different insects using the Anopheles gambiae genome as the reference. Solid horizontal lines represent the chromosome or scaffold. Orthologue genes are represented by the swallowtail symbols with the same colour (numbers represent gene IDs (The prefix is AGAP0061)). The small triangles linked with the swallowtail represent intron-located genes. The tip of the swallowtail symbols indicates the direction of transcription. Black and white interval lines represent the insertion of multiple genes