Fig. 1
Plasma (a) and egg-yolk (b) IgY concentrations in uninfected or experimentally infected birds in two experiments. Infected birds received an oral inoculation of 1,000 embryonated eggs of A. galli or H. gallinarum and necropsied 28 or 30 week p.i., respectively. Data are shown with the outlier box plots with the vertical line within the box representing the sample median, and the lower and upper end of the box representing the 25th and 75th quantiles, respectively. Each dot is an independent observation. The extent of the whiskers is calculated as 25th and 75th quartiles plus or minus 1.5 × interquartile range, respectively. Outliers above the grey line (* > 300) are shown on an extended reduced scale [reduced y-axis = (y*0.01 + 300)] to depict a focused picture of the whole data. The dashed red lines indicate the cut-off values quantified by the ROC analyses for each nematode and sample matrix (see Table 2 for details). The figure represents raw data, but the statistical comparisons are based on the log-transformed data