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Fig. 7 | Parasites & Vectors

Fig. 7

From: Ultrastructure and localization of Neorickettsia in adult digenean trematodes provides novel insights into helminth-endobacteria interaction

Fig. 7

Overview of immunohistological detection of endobacteria in adult P. elegans using pre-immune serum (a) or polyclonal antisera against rPeNsp-3 (b-e). a Cross-section of the proximal part of the worm labeled with a pre-immune serum as negative control. Plasma membranes are stained in red and nuclei are stained in blue (DAPI). b Consecutive section to a stained for PeNsp-3. Note clusters of green endobacteria (arrows) in the tegument, the oral sucker and the ootype. c Cross-section of the region of the ventral sucker (compare Additional file 1: Figure S1) of an adult P. elegans in the intestine of a hamster stained for PeNsp-3. Numerous individual or small clusters of green-labeled endobacteria are seen in various worm tissues especially the tegument, the gut epithelium, ootype, vitteline glands and Mehlis’ gland. Note the green staining in the hamster gut (arrows). d Another cross-section of the mid-body region showing endobacteria also in the anterior testis and the epithelium of the excretory bladder. Again green labeling can be seen in hamster gut (arrow). e Cross-section of the distal part of an adult worm showing many endobacteria in the posterior testis, but only a few scattered endobacteria in the intrauterine eggs. Like in c and d green labeling of clustered endobacteria (arrow) can be detected occasionally in the hamster gut. Abbreviations: ate, anterior testis; eb, excretory bladder; e, egg; g, gut; hg, hamster gut; Mg, Mehlis’gland; os, oral sucker; ot, ootype; ov, ovary; ph, pharynx; pte, posterior testis; ts, tegumental syncytium; ut, uterus; vf, vitelline follicle; vs, ventral sucker;. Scale-bars: 100 μm

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