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Fig. 1 | Parasites & Vectors

Fig. 1

From: Leishmania major large RAB GTPase is highly immunogenic in individuals immune to cutaneous and visceral leishmaniasis

Fig. 1

Expression of recombinant rLmlRAB and rLmlRABC in E. coli. Recombinant rLmlRAB as well as its carboxyl terminal part rLmlRABC were synthesized in BL21, purified by affinity chromatography over Ni-NTA resin. Western blot analysis shows the reactivity of the polyclonal anti-LmlRAB against the insoluble fraction of E. coli expressing both proteins rLmlRAB (a Lane 1) and LmlRABC (b Lane 1). The purified proteins rLmlRAB (a Lane 2) and rLmlRAB (b Lane 2) were also analyzed

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