Fig. 3

rLmlRAB and rLmlRABC specific IFN-γ, granzyme B, IL-10 and TNF-α responses. a IFN-γ, b granzyme B, c IL-10 and d TNF-α were detected and quantified from culture supernatants of PBMC (106 cells/ml) stimulated for 120 h with SLA (10 μg/ml), rLmlRAB or rLmlRABC (10 μg/ml). IFN-γ and IL-10 production were measured by ELISA. Granzyme B and TNF-α were measured using CBA and data were analyzed by flow cytometry. PHA (10 μg/ml) was used as positive control (data not shown). Bars indicate the median value for IFN-γ (a) and IL-10 (c) and the mean value for granzyme B (b) and TNF-α (d). Statistically significant differences were only shown for rLmlRAB or rLmlRABC, and were performed in each group and between groups (P ≤ 0.01)