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Table 2 Primers used for cloning of LmlRAB and LmlRABC and plasmid constructions

From: Leishmania major large RAB GTPase is highly immunogenic in individuals immune to cutaneous and visceral leishmaniasis

Name Type Primer sequences Primer sequences Restriction site
F-Ras1a Forward rlmlrab 5′-GCCCATATGAGCTCAACTGGTCAGCATG-3′ NdeI
F-Ras3a Forward rlmlrabC 5′GCCCATATGATCATCGCCGATGTGTCG-3′ NdeI
R-Ras3b Reverse rlmlrab/rlmlrabC 5′-GCCGCGGCCGCCCAGTACGCAGCAGTTGC-3′ NotI
  1. Reverse and forward primers containing at their 5′ end a restriction site (underlined) were used to amplify the full length LmlRAB ORF and its carboxyl part LmlRABC. The amplified double-stranded DNA were first digested with NdeI and NotI and then inserted into the corresponding cloning sites in the pET-22b + vector (Novagen)