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Table 1 Description of the primers and amplification conditions of the protocol by Santos et al. [15], performed for molecular detection of Plasmodium spp. based on 18S rRNA gene

From: Molecular identification of Plasmodium spp. and blood meal sources of anophelines in environmental reserves on São Luís Island, state of Maranhão, Brazil

Primer Primer sequence/amplification conditions Product size (bp)
rPLU6R 5'-CGTTTTAACTGCAACAATTTTAA-3' 600
rPLU1 5'-TCAAAGATTAAGCCATGCAAGTGA 3'  
1st reaction 95 °C for 5 min; 25 cycles of 95 °C for 1 min, 58 °C for 2 min, 72 °C for 2 min; and a final extension step at 72 °C for 5 min  
rPLU3 rPLU4 5'-TTTTTATAAGGATAACTACGGAAAAGCTGT-3' 5'-TACCCGTCATAGCCATGTTAGGCCAATACC-3' 240
2nd reaction 95 °C for 5 min; 30 cycles of 95 °C for 1 min, 64 °C for 2 min and 72 °C for 2 min; and a final extension at 72 °C for 5 min