Primer | Primer sequence/amplification conditions | Product size (bp) |
---|---|---|
rPLU6R | 5'-CGTTTTAACTGCAACAATTTTAA-3' | 600 |
rPLU1 | 5'-TCAAAGATTAAGCCATGCAAGTGA 3' | |
1st reaction | 95 °C for 5 min; 25 cycles of 95 °C for 1 min, 58 °C for 2 min, 72 °C for 2 min; and a final extension step at 72 °C for 5 min | |
rPLU3 rPLU4 | 5'-TTTTTATAAGGATAACTACGGAAAAGCTGT-3' 5'-TACCCGTCATAGCCATGTTAGGCCAATACC-3' | 240 |
2nd reaction | 95 °C for 5 min; 30 cycles of 95 °C for 1 min, 64 °C for 2 min and 72 °C for 2 min; and a final extension at 72 °C for 5 min |