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Table 2 Description of the primers, probe and amplification conditions of the qPCR protocol by Lima et al. [22], performed for molecular detection of Plasmodium spp. based on 18S rRNA gene

From: Molecular identification of Plasmodium spp. and blood meal sources of anophelines in environmental reserves on São Luís Island, state of Maranhão, Brazil

Primer/probe Primer sequence/amplification conditions Product size (bp)
M60 5'-ACA TGG CTA TGA CGG GTA ACG-3' 84
M61 5'-TGC CTT CCT ATG TAG TGG TAG CTA-3'  
M62 5'-FAM TCA GGC TCC CTC TCC GGA ATC GA-TAMRA-3'  
Reaction 50 °C for 2 min and 95 °C for 10 min; 40 cycles of 94 °C for 30 s and 60 °C for 1 min