Table 2 Description of the primers, probe and amplification conditions of the qPCR protocol by Lima et al. [22], performed for molecular detection of Plasmodium spp. based on 18S rRNA gene
Primer/probe | Primer sequence/amplification conditions | Product size (bp) |
|---|---|---|
M60 | 5'-ACA TGG CTA TGA CGG GTA ACG-3' | 84 |
M61 | 5'-TGC CTT CCT ATG TAG TGG TAG CTA-3' | |
M62 | 5'-FAM TCA GGC TCC CTC TCC GGA ATC GA-TAMRA-3' | |
Reaction | 50 °C for 2 min and 95 °C for 10 min; 40 cycles of 94 °C for 30 s and 60 °C for 1 min |