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Table 3 Sequences of primers based on the cytochrome b (cytb) gene that were used to identify the food sources of anopheline mosquitos [24]

From: Molecular identification of Plasmodium spp. and blood meal sources of anophelines in environmental reserves on São Luís Island, state of Maranhão, Brazil

Food source tested

Primer sequence

Product size (bp)

Domestic cat

TTCTCAGGATATACCCTTGACA

180

GAAAGAGCCCATTGAGGAAATC

Human

TTCGGCGCATGAGCTGGAGTCC

228

GTRTARTAGGGRTGRAATC

Domestic dog

GAACTAGGTCAGCCCGGTACTT

153

CGGAGCACCAATTATTAACGGC

Birds

GACTGTGACAAAATCCCNTTCCA

508

GTCTTCATCTYHGGYTTACAAGAC

Reaction

94 °C for 2 min; 35 cycles of 94 °C for 30 s, 54–70 °Ca for 30 s, 72 °C for 30 s; and a final extension step at 72 °C for 20 min

 
  1. aDepending on the annealing temperature of each oligonucleotide (see Chang et al. [24])