Mining a differential sialotranscriptome of Rhipicephalus microplus guides antigen discovery to formulate a vaccine that reduces tick infestations

Table 1 Functional classification of putative secreted proteins in the RMallHxN dataset comprising eight libraries (UFLRmS, UFLRmR, SGNRmS, SGNRmR, SGMRmS, SGMRmR, SGFRmS and SGFRmR)

Functional class	Total contigs	Total ESTs	% of total
Related to host immunity^a	12	23	0.29
TIL domain	16	34	0.43
Proteinase inhibitors
Kunitz domain	30	87	1.10
Serpin	1	2	0.03
Thyropin domains	1	2	0.03
Cystatin	1	1	0.01
Carboxypeptidase inhibitor	2	3	0.04
Lipocalins/histamine binding proteins	35	61	0.77
Immunoglobulin binding proteins	4	57	0.72
Mucins	13	31	0.39
Basic tail family	10	22	0.28
Enzymes
Metalloproteases	20	48	0.61
Calpain	6	7	0.09
Serine protease	10	42	0.53
Serine carboxypeptidases	2	2	0.03
Antigen 5 family	4	23	0.29
Glycine rich family
Cement proteins	112	1024	12.92
GYY family	15	70	0.88
GGY family	4	8	0.10
Other secreted proteins	12	13	0.16
R. microplus-specific proteins	107	296	3.74
Unknown secreted	2,082	2,836	35.79

^aThese transcripts encoding proteins that may play role in modulation of host immunity. They were classified as “similar to protein associated with interferon”, “microplosin family”, “DAP-36 immunosuppressant family”, “Evasin”, “Defensin” and “Ixoderin” as displayed on Additional file 1

ISSN: 1756-3305