Fig. 3
From: Characterization of a novel aspartyl protease inhibitor from Haemonchus contortus
Localization of API in adult worms. Cryostal sections of H. contortus were incubated with rat antibody against the recombinant API, following with the second antibody Cy3-labeled Goat Anti-Rat IgG. The red fluorescence was detected in both female (a, d) and male (b, e) adult H. contortus, but no fluorescence was observed in control experiments (c, f). To see the distributions of the API, vertical (a, b, c) and transverse sections (d, e, f) were checked. Images in lines 1 and 2 were taken under UV light and white field; merged images are given in line 3. Scale-bars: 100 μm