Fig. 5
From: Characterization of a novel aspartyl protease inhibitor from Haemonchus contortus
Inhibition assay of API on pepsin and trypsin. The products of hemoglobin (BSA) digested by pepsin (trypsin) with (or without) API were analysed by SDS-PAGE. Lane 1: hemoglobin; Lane 2: pepsin; Lane 3: API; Lane 4: pepsin + hemoglobin; Lane 5, pepsin incubated with API for 30 min (at 37 °C, pH 4.0) + hemoglobin; Lane 6: BSA; Lane 7: trypsin; Lane 8: trypsin + BSA; Lane 9: trypsin incubated with API for 30 min (at 37 °C, pH 4.0) + BSA