Fig. 3
From: Geno- and phenotypic characteristics of a transfected Babesia bovis 6-Cys-E knockout clonal line
PCR analysis for integration of egfp-bsd in transfected B. bovis. a Top panel: schematic representation of the intact 6-Cys E locus in the T3Bo strain and the 6-Cys EKO-cln parasites as deduced from full genome sequence of 6-Cys EKO-cln parasites. The red coloured lines represent gDNA sequences that are not a part of the targeted region included in the transfection plasmid p6-Cys-EKO. Lower panel: agarose electrophoresis analysis performed on the PCR products corresponding to each of the fragments numbers 1–7 in the upper panel. b Comparative PCR analysis performed on the line EKO-cln, T3Bo, and EKO, using RAP-1, 6-Cys E, egfpbsd, and IGE-Pro primer sets. SM represents standard molecular weight 10 Kbp