Prevalence of tick-borne haemoparasites in small ruminants in Turkey and diagnostic sensitivity of single-PCR and RLB

Table 2 Comparison of PCR and RLB results

Species			PCR		Total	P-value	Measurement of agreement^a
Species			Positive	Negative	Total	P-value	Kappa value	SD (95% CI)
A. ovis	RLB	Positive	693	120	813	< 0.0001*	0.344	0.019
	RLB	Negative	560	606	1166
	Total		1253	726	1979
T. ovis	RLB	Positive	1065	123	1188	< 0.0001*	0.710	0.016
	RLB	Negative	150	641	791
	Total		1215	764	1979
T. luwenshuni	RLB	Positive	1	0	1	< 0.0001*	0.012	0.012
	RLB	Negative	148	1830	1978
	Total		149	1830	1979
T. uilenbergi	RLB	Positive	0	10	10	0.404	-0.009	0.003
	RLB	Negative	128	1841	1969
	Total		128	1851	1979
Theileria sp. MK	RLB	Positive	6	1	7	< 0.0001*	0.179	0.064
	RLB	Negative	52	1920	1972
	Total		58	1921	1979
B. ovis	RLB	Positive	5	3	8	< 0.0001*	0.081	0.035
	RLB	Negative	101	1870	1971
	Total		106	1873	1979
A. phagocytophilum	RLB	Positive	0	0	0	–	–	–
	RLB	Negative	31	1948	1979
	Total		31	1948	1979

*P-values considered as statistically significant (P < 0.05) based on the Chi-square test
^aAgreement expressed as kappa value when comparing PCR and RLB tests for A. ovis, T. ovis, T. luwenshuni, T. uilenbergi, Theileria sp. MK, B. ovis and A. phagocytophilum two-by-two. None of the collected samples were found to be positive for T. lestoquardi by PCR and RLB

ISSN: 1756-3305