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Table 2 Comparison of PCR and RLB results

From: Prevalence of tick-borne haemoparasites in small ruminants in Turkey and diagnostic sensitivity of single-PCR and RLB

Species    PCR Total P-value Measurement of agreementa
Positive Negative Kappa value SD (95% CI)
A. ovis RLB Positive 693 120 813 < 0.0001* 0.344 0.019
Negative 560 606 1166    
Total 1253 726 1979    
T. ovis RLB Positive 1065 123 1188 < 0.0001* 0.710 0.016
Negative 150 641 791    
Total 1215 764 1979    
T. luwenshuni RLB Positive 1 0 1 < 0.0001* 0.012 0.012
Negative 148 1830 1978    
Total 149 1830 1979    
T. uilenbergi RLB Positive 0 10 10 0.404 -0.009 0.003
Negative 128 1841 1969    
Total 128 1851 1979    
Theileria sp. MK RLB Positive 6 1 7 < 0.0001* 0.179 0.064
Negative 52 1920 1972    
Total 58 1921 1979    
B. ovis RLB Positive 5 3 8 < 0.0001* 0.081 0.035
Negative 101 1870 1971    
Total 106 1873 1979    
A. phagocytophilum RLB Positive 0 0 0
Negative 31 1948 1979    
Total 31 1948 1979    
  1. *P-values considered as statistically significant (P < 0.05) based on the Chi-square test
  2. aAgreement expressed as kappa value when comparing PCR and RLB tests for A. ovis, T. ovis, T. luwenshuni, T. uilenbergi, Theileria sp. MK, B. ovis and A. phagocytophilum two-by-two. None of the collected samples were found to be positive for T. lestoquardi by PCR and RLB